Plasmolysis is a shrinkage of protoplast from the cell wall under the influence of hypertonic. solution. This can be observed by placing of fresh filament of Spirogyra or fresh peel from the lower surface of Rhoeo (= Tradescantia) in a 10% solution of common salt. The cells undergo exosmosis. Loss of water results in shrinkage of protoplast accompanied by shrinkage of cell size. Later protoplast will separate from the wall first at the corners (incipient plasmolysis) and then from other parts (evident plasmolysis) except for certain points in the region of plasmodesmata, if present.