Question

You are given a permanent slide of budding in Yeast for observation under a compound
microscope. Following are the four steps of focussing the object to be observed under
the microscope.
(I) Look through the eye piece and raise the objective using coarse adjustment until
the object is focussed.
(II) Place the slide on the stage ; look though the eye piece and adjust the mirror and
diaphragm to illuminate the object.
(III) Look through the eye piece and move the slide until the object is visible.
(IV) Make the focus sharp with the help of fine adjustment.
Arrange these steps in proper sequence

• A. (I), (III), (II), (IV)
• B. (III), (II), (I), (IV)
• C. (II), (III), (IV), (I)
• D. (II), (III), (I), (IV)

Answer 1

The correct option is D (II), (III), (I), (IV)

To observe an object under a microscope, we have to place the slide on the stage,look through the eye piece and adjust the mirror and diaphragm to illuminate or properly place under the light of the microscope->then we will have to look through the eye piece by placing our one eye properly and move the slide until the object is visible properly->next we have to keep staring at the object through the eye piece turning the knobs that adjusts the distance between the stage and the lens,coarse adjustment is used to bring the specimen into initial focus to be visible but not sharp->then the fine adjustment is adjusted to make the specimen focus and sharply visible.

So,the correct option is (D).