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B
Three layered
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C
Two layered
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D
One layered
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Solution
The correct option is C Three layered
Zygospore in Spirogyra is three-layered with a brownish-orange colored outer layer.
For germination, the zygospore nucleus divides meiotically and out of four daughter nuclei formed, three disintegrate, leaving only one haploid nucleus. Hence, Zygospore of Spirogyra is Three-layered.