Question

In reverse phase chromatography, the stationary phase is made:-

• A. Polar
• B. Non-Polar
• C. Either Polar Or Non-Polar
• D. None of these

Answer 1

The correct option is B

Non-Polar

The correct option is B.

Explanation of the correct option:

1. A hydrophobic or nonpolar stationary phase and a hydrophilic or polar mobile phase are used in reversed-phase liquid chromatography (RPLC).
2. It involves the separation of molecules on the basis of hydrophobic interactions occurring between the solute molecules in the mobile phase and the ligands attached to the stationary phase.
3. Porous silica particles coupled to alkyl chains (C4, C5, C8, C18) or other inert nonpolar compounds such as divinyl benzene are typically used as the stationary phase (DVB).

Explanation of the incorrect options:

Option A:-

1. The stationary phase in normal-phase chromatography is polar, while the mobile phase is nonpolar.
2. In reversed phase, the stationary phase is nonpolar while the mobile phase is polar.

Option C:-

1. In reverse phase chromatography, the stationary phase cannot be either in the polar or non-polar stage.

Final Answer: - In reverse phase chromatography, the stationary phase is made non-polar.