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Question

What are the four stages for DNA preparation from a culture of bacterial cells?


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Solution

DNA preparation:

  1. There are 3 basic phases involved in DNA extraction, that is, lysis, precipitation, and purification.
  2. DNA is released during lysis because the cell and nucleus are split open.
  3. The cellular proteins and free DNA are dissolved during this mechanical disruption process using enzymes and detergents like Proteinase K.

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