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Electroporation

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Question

Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombined technology experiments.

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Solution

Four ways to introduce the desired DNA segment into the bacterial host are:
Heat shock based transformation
Electroporation
Using disarmed bacteriophages as vectors (Transduction)
Micro-injection.

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Q. Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.

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Q. Given below are the steps of recombinant DNA technology.

(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host

Choose the correct sequence of events.

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a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
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d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

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