Question

5'-GAATTC-3' is the recognition site, for which of the following restriction endonuclease?

• A. Hind III
• B. EcoRI
• C. Bam I
• D. Hae III

Answer 1

The correct option is B EcoRI

• EcoRI is the restriction enzyme, derived from Escherichia coli,strain R and is the first to be isolated.
• EcoRI recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends.
• This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.

Thus, the correct answer is option B.