Molecular Diagnosis

Q. Which of these is true about Molecular Diagnosis

1. All of the above
2. These diagnostic methods have exisited for over a century
3. It helps in early detection of disease
4. The procedures take a painstakingly long time

Q.

Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the options given.

I. Electrophoresis of DNA fragments.

II. Hybridisation with DNA probe.

III. Digestion of DNA of RENs.

IV. Autoradiography.

V. Blotting of DNA fragments to nitrocelluose membrane.

1. III, I, V, II, IV

2. I, III, V, IV, II

3. III, I, II, V, IV

4. I, V, III, II, IV

Q.

Mention the steps involved in DNA fingerprinting

Q.

What is it that forms the basis of DNA fingerprinting?

1. The relative difference in the DNA occurrence in blood, skin and saliva

2. The relative amount of DNA in the ridges and grooves of the fingerprints.

3. The relative proportions of purines and pyrimidines in DNA

4. Satellite DNA occurring as highly repeated short DNA segments

Q.

What is Quantitative PCR?

Q.

How does dot blot start?

Q.

How can the fluorescence intensity be measured?

Q.

The basis of DNA fingerprinting is:

1. The double helix

2. Errors in the base sequence

3. ​​​​​​Polymorphism in the sequence

4. DNA replication

Q.

Blood stains are found at the site of the murder. If DNA profiling technique is to be used for identifying the criminal which of the following is ideal for use?

1. erythrocytes

2. leucocytes

3. platelets

4. serum

Q. Which of these can be called as Molecular diagnosis method(s)

1. PCR
2. ELISA
3. Angiogram
4. DNA Microarray

Q.

DNA fingerprinting refers to

1. molecular analysis of profiles of DNA samples

2. analysis of DNA samples using imprinting device

3. techniques used for molecular analysis of different specimens of DNA

4. techniques used for identification of fingerprints of individuals

Q. Which of these is true about Molecular Diagnosis

1. These diagnostic methods have exisited for over a century
2. The procedures take a painstakingly long time
3. It helps in early detection of disease
4. All of the above