Tissue Culture

Q.

What is the meaning of Plant Tissue Culture???

Q. What are the advantage of tissue culture

Q.

Where Are Cytokinins Produced?

Q.

How are plants cloned using micropropagation?

Q.

Sterilization of tissue culture medium is done by:

1. Keeping the medium at -20°C

2. Filtering the medium through a fine sieve.

3. Mixing the medium with antifungal agents.

4. Autoclaving of the medium at 120°C for 15 minutes.

Q.

What are the effects of cytokinins?

Q.

In plant tissue culture, new plants be produced from leaves, anther, ovary or root. What could be the reason?

1. plant cells give rise to new plants by hormonal affect

2. genes are stimulated for giving rise to new plants

3. all plant cells are totipotent

4. special nutrients supplied to plant parts produce new plants

Q. What is meant by grafting?

Q.

Plant tissue culture is a method of ______.

1. hybridisation

2. vegetative propagation

3. sexual reproduction

4. mushroom cultivation

Q. ________ is a artificial method of cultivation of plants in a sterile and nutritive medium.

1. Tissue culture
2. Layering
3. Grafting
4. Cutting

Q. What is plant tissue culture? What are the important steps involved in it?

Q. The best way to obtain bacterial and viruses free plants through tissue culture

1. Micropropagation
2. Seed germination after gamma-irradiation
3. Seed germination under aseptic condition
4. Stem or Shoot up culture

Q. The technique of producing large number of genetically similar plants, within short time by tissue culture is called as

1. Organogenesis
2. Somatic hybridization
3. Micropropagation
4. Protoplast culture

Q.

Explain the biological activity of cytokinins.

Q. Some leaves are removed from the stem cutting planted for vegetative propagation. This is done

1. To increase water uptake
2. Because it helps in rooting of cutting
3. To reduce water loss
4. Because the cutting need less food

Q.

How were gibberellins first discovered.

Q.

Based on the image, choose the correct option.

1. 1 - Explant, 2 - Callus, 3 - Organogenesis, 4 - Plant tissue culture
2. 1 - Explant, 2 - Callus, 3 - Somatic embryogenesis, 4 - Regeneration
3. 1 - Explant, 2 - Callus, 3 - Somatic embryogenesis, 4 - Plant tissue culture
4. 1 - Explant, 2 - Organogenesis, 3 - Regeneration, 4 - Plant tissue culture

Q. A plant hormone that induces morphogenesis in plant tissue culture is

1. Abscisic acid
2. Gibberellin
3. Cytokinin
4. Ethylene

Q. What is incorrect about tissue culture?

1. It has to be done in sterile conditions
2. It is done in a laboratory
3. It is started with a very small part of the parent plant
4. It's end result is a shapeless mass of cells

Q.

Some plants have two or more edible parts. True or false?

1. True
2. False

Q. What is embryo rescue? When is this method followed?

Q. The production of new plants by cells instead of seeds is done by

1. Amphimixis
2. Biofertilizers
3. Parthenocarpy
4. Tissue culture

Q. Stem cuttings are often treated with NAA before sowing in order to promote

1. Sprouting of buds
2. Rooting
3. Layering
4. Development of adventitious buds

Q. Select the correct set.

Column I	Column II
I. Bamboos	A. Bronchial allergy
II. Microspore	B. Die after flowering
III. Cleistogamous	C. Orchids
IV. Micropropagation	D. Flowers which never open

1. I - A, II - B, III - C, IV - D
2. I - B, II - A, III - D, IV - C
3. I - A, II - D, III - B, IV - C
4. I - D, II - B, III - C, IV - A

Q. Sterilization of tissue culture medium is done by

1. Filtering the medium through fine sieve
2. Autoclaving of medium at ${120}^0$C for 15 minutes
3. Mixing the medium with antifungal agents
4. Keeping the medium at $-{20}^{0}C$

Q.

What is incorrect about tissue culture?

1. It has to be done in sterile conditions

2. It is done in a laboratory.

3. It is done with a very small part of the parent plant.

4. Its end result is a shapeless mass of cells.

Q. What do you understand by micropropagation?

Q. To meet the demands of the society, in vitro production of a large number of plantlets in a short duration is practiced in floriculture and horticulture industry today. This is called

1. Micropropagation
2. Hybridoma technology
3. Somatic hybridisation
4. Somaclonal variation

Q. maam 1 more query maam does tissue culture can help us in the formation of viruses artifically like in case of corona virus?

Q.

Which one of the following statements can best explain the term critical concentration of an essential element?

1. Essential element concentration below which plant remains in the vegetative phase.

2. Essential element concentration below which plant dies.

3. Essential element concentration below which plant growth is retarded.

4. Essential element concentration below which plant growth is faster.