Gene Cloning

Q.

How long does DNA replication take?

Q. Which of the following statements are correct regarding sexual reproduction?

1. Offsprings are always identical to parents
2. All the variations are useful for an organism to survive
3. Offsprings show some variation from the parents
4. Reproduction ensures continuity of species

Q. Explain how in sexually reproducing animals their number of progeny is maintained.
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Q.

What is the role of cloning vector?

Q. mRNA is synthesised on DNA template in which direction:

1. Both
2. $5^{\prime }\to 3^{\prime }$
3. $3^{\prime }\to 5^{\prime }$
4. Any

Q.

An offspring can also be called:

1. Variety

2. Progeny

3. Clone

4. Parent

Q.

Given below is the image depicting the steps involved in the manufacture of insulin using bacteria through genetic engineering. Match the numbers given in the image with the steps given below.

A. Joining the two DNA segments

B. Multiplication of bacteria

C. Identification of insulin gene

D. Insertion of the new genome into bacteria

E. Cutting the gene and bacterial genome

1. 1 $\Rightarrow$ A, 2 $\Rightarrow$ B, 3$\Rightarrow$ C, 4$\Rightarrow$ D, 5$\Rightarrow$ E

2. 1 $\Rightarrow$ C, 2 $\Rightarrow$ E, 3$\Rightarrow$ A, 4$\Rightarrow$ D, 5$\Rightarrow$ B

3. 1 $\Rightarrow$ E, 2 $\Rightarrow$ D, 3$\Rightarrow$ C, 4$\Rightarrow$ B, 5$\Rightarrow$ A

4. 1 $\Rightarrow$ B, 2 $\Rightarrow$ C, 3$\Rightarrow$ D, 4$\Rightarrow$ E, 5$\Rightarrow$ A

Q.

What was developed by Dr. Ian Wilmut and his colleagues in roselind institute in scotland in july 1996?

Q. Antibiotic resistance is passed from one bacterium to the other by the process of ______.

1. Chemotherapy
2. Cloning
3. Restriction digestion
4. Conjugation

Q.

Match the substages of prophase I of first meiotic division listed in column I with the important events pertaining to respective stages given under column II.

$\begin{array}{cccc}& ColumnISubstagesofprophaseI& & ColumnIIImportantevents\\ A& \text{Zygotene}& p& \text{Chromomeres}\\ B& \text{Pachytene}& q& \text{Bivalents migrate to periphery}\\ C& \text{Diplotene}& r& \text{Terminalization of chiasmata begins}\\ D& \text{Diakinesis}& s& \text{Crossing over}\\ & & t& \text{Synapsis}\end{array}$

1. A = p, B = s, C = r, D = q

2. A = t, B = s, C = r, D = q

3. A = s, B = p, C = r, D = q

4. A = s, B = t, C = r, D = q

Q. A sheep Dolly was the first animal cloned from a cell taken from the udder of an adult transgenic sheep.

1. true
2. false

Q. What is cloning?

Q.

The first cloning was done on

1. Goat

2. Sheep

3. Cow

4. Deer

Q. The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C.
$DNA\stackrel{A}{\to }mRNA\stackrel{B}{\to }protein$
This was proposed by C.

1. A-translation B-transcription C-Erevin Chargaff
2. A-transcription B-translation C-Francis Crick
3. A-transcription B-replication C-James Watson
4. A-translation B-extension C-Rosalind Franklin

Q. Identify A and B in the given table.

$\begin{array}{cc}\text{Technique}& \text{Usage}\\ \text{DNA cleaving}& \text{used for cutting genes at special sites}\\ \text{(A) \_\_\_\_\_\_\_\_\_}& \text{used for testing the arrangement of nucleotide}\\ \text{Gene therapy}& \text{used for treating genetic diseases}\\ \text{(B) \_\_\_\_\_\_\_\_\_}& \text{used for locating the position of a gene}\end{array}$

1. A - Gene mapping, B - DNA profiling
2. A - DNA profiling, B - Gene mapping
3. A - Ligase, B - Gene mapping
4. A - DNA profiling, B - Ligase

Q. Clone Dolly was produced by the attempts of

1. Charles Darwin
2. Ian Wilmut
3. Lederberg
4. None of these

Q. Which one of the following is used as vector for cloning genes into higher organisms?

1. Retrovirus
2. Baculovirus
3. Rhizopus nigricans
4. Salmonella typhimurium

Q. Genetic factors are a major cause of autoimmune diseases.

1. atrichous
2. monotrichous

Q. Which of the following statements is not true of two genes that show $50\%$ recombination frequency?

1. The genes may be on different chromosomes
2. The genes are tighly linked
3. The genes show independent assortment
4. If the genes are present on the same chromosome, they undergo more than one crossovers in every meiosis

Q. 'Cloning' is meant

1. For production of HGH gene is E. coli
2. To preserve the genotype of organism
3. To replace the original gene
4. All of the above

Q. What are somaclones?

Q. Cloning of animals has been possible due to advances in

1. nuclear technology
2. computer technology
3. biotechnology
4. autoradiography

Q. Population of cells derived from a single parent cell is called

1. Diploid
2. Haploid
3. Clone
4. Symmetrical cell

Q. A population of genetically identical individuals, obtained from asexual reproduction is

1. Callus
2. Clone
3. Deme
4. Aggregate

Q.

Identify the component marked '?' in the following image.

1. Restriction Endonuclease

2. Exonuclease

3. Ligase

4. Amylase

Q. Name the causative organism of the disease Malaria and its mode of asexual reproduction.

Q. Genetic and acquired diseases can be cured by cloning.

1. True
2. False

Q. A clone is

1. Heterozygote obtained asexually
2. Homozygote produced by sexual reproduction
3. Heterozygote produced by sexual methods
4. Homozygote obtained asexually

Q. Assertion :A lock of Einstein's hair, if scientists could locate it and extract its DNA, could help in producing another Einstein, by cloning. Reason: The DNA extracted from the cell of an embryo at an early stage of development can be transferred to individual eggs which can be implanted into the uterus of a surrogate mother to give birth to an identical offspring.

1. Both A and R are true but R is the correct explanation of A
2. Both A and R are true but R is not a correct explanation of A
3. A is true but R is false
4. A is false but R is true

Q. A sheep named Dolly became famous because of

1. Embryo rescue
2. Cloning
3. In vitro fertilization
4. Transgenic animal