Bacterial Synthesis of Insulin (Human)

Q.

Identify the substances having glycosidic bond and peptide bond, respectively in their structure.

1. Cellulose, lecithin

2. Inulin, Insulin

3. Chitin, cholesterol

4. Glycerol, trypsin

Q.

Insulin is secreted by

1. beta cells of pancreas

2. alpha cells of pancreas

3. delta cells of pancreas

4. none of these

Q. The regulation of the lac operon is referred as negative regulation because:

1. the regulatory molecule stops the transcription of structural genes
2. it gets activated only in the presence of lactose
3. the products of the structural genes digest lactose

4. All the above statements are correct

Q.

Which two hormones are secreted by pancreas? Write any one function of each of these hormones.

Q.

The deficiency of insulin causes _____.

Q.

What hormone raises blood sugar?

Q. Humulin is

1. A powerful antibiotic
2. A form of chitin
3. Human insulin
4. A new digestive enzyme

Q. Identify A and B in the figure given below.

1. A: Proinsulin, B: C peptide
2. A: C peptide, B: Proinsulin
3. A: Insulin, B: C peptide
4. A: Insulin, B: Proinsulin

Q. Humulin is

1. A form of chitin
2. A powerful antibiotic
3. A new digestive enzyme
4. Human insulin

Q.

During bacterial synthesis of insulin, the plasmid containing the insulin gene is introduced into the

1. None of these
2. Pneumococcus
3. E.coli
4. Bt

Q. What is the importance of biotechnology application in medicine? How does biotechnology help in the production of human insulin?

Q.

Human insulin is being commercially produced from a transgenic species:

1. Rhizobium

2. Saccharomyces

3. Escherichia

4. Mycobacterium

Q. Read the following statements carefully and select the correct option.
Statement I: Insulin is produced by alpha cells of islets of Langerhans.
Statement II: During maturation of insulin from proinsulin, C peptide is removed for proper folding.

1. Both I and II are true
2. I is true but II is false
3. I is false but II is true
4. Both I and II are false

Q. Select the correct order of the components of Lac operon in E. coli.

1. Pi → i → P → O → LacZ → LacY → LacA

2. Pi → i → O → P → LacZ → LacY → LacA

3. Pi → i → P → O → LacZ → LacA → LacY
4. Pi → i → O → p → LacZ → LacA → LacY

Q. The term biotechnology was used for the first time by

1. Craig Venter
2. Karl Ereky
3. Stanley Cohen
4. Herbert Boyer

Q. Which of the following statements regarding the structure of proinsulin and mature insulin are not correct?
(i) Proinsulin is made up of three polypeptide chains A, B, and C.
(ii) C-polypeptide chain with 33 amino acids is removed prior to insulin formation.
(iii) Mature insulin is made up of 51 amino acids and arranged in two polypeptide chains-A and B.
(iv) Polypeptide chain A has 30 amino acids and polypeptide chain B has 21 amino acids.
(v) Polypeptide chains A and B are interconnected by only one S-S linkage.

1. (i) and (ii)
2. (iii) and (iv)
3. (iv) and (v)
4. (iii), (iv) and (v)

Q. Read the following statements carefully and select the correct option.
Statement I: Insulin is produced by alpha cells of islets of Langerhans.
Statement II: During maturation of insulin from proinsulin, C peptide is removed for proper folding.

1. Both I and II are true
2. I is true but II is false
3. I is false but II is true
4. Both I and II are false

Q. Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bio reactors.
(iii) Insertion of human insulin gene into a plasmid.

1. (i), (iii), (iv), (ii), (v)
2. (iii), (v), (ii), (i), (iv)
3. (ii), (i), (iv), (iii), (v)
4. (i), (iii), (v), (ii), (iv)

Q. Insulin is secreted by:

1. Beta cells of Islets of Langerhans
2. Alpha cells of Islets of Langarhans
3. Kupffer cells
4. Gall bladder

Q. Which of the following represents the correct sequence of genes of a lac operon system?

1. Promoter gene, terminator sequence, structural genes
2. Promoter gene, structural genes, operator gene
3. Promoter gene, operator gene, structural genes
4. Structural genes, promoter gene, operator gene

Q. The term ‘Humulin’ is used for

1. Hydrolytic enzyme
2. Isoenzyme
3. Human insulin
4. Antibiotic

Q. Some of the steps involved in the production of humulin are given. Choose the correct sequence (i) Synthesis of gene (DNA) for human insulin artificially (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.

1. ii, i, iv, iii, v, vi
2. i, iii, v, vi, ii, iv
3. i, iv, v, ii, vi, iii
4. iii, v, ii, i, vi, iv

Q. How did an American company, Eli Lilly use the knowledge of rDNA technology to produce human insulin?

Q. (a) How is mature insulin different from proinsulin secreted by pancreas in humans? (b) Explain how was human functional insulin produced using rDNA technology (C) Why is , the functional insulin produced considered better than the ones used earlier by diabetic patients?

Q.

Match the hormones mentioned in column I with the functions listed in column II and choose the right answer containing the correct matching of the alphabets of the two columns:

Column I - Hormones	Column II - Function
A. LTH (or PL)	e. Promotes growth
B. TSH	f. Elevates BP
C. GH (or STH)	g. Stimulates milk secretion
	h. Stimulates secretion of thyroxine

1. A=e, B= f, C= g

2. A=g, B= h, C= e

3. A= h, B= g, C= f

4. A= f, B= e, C= g

Q. Would the insulin isolated from other animals be just as effective as that secreted by the human body itself?

Q. What is used in production of insulin by genetic engineering?

1. Rhizobium
2. Saccharomyces
   
3. Mycobacterium
4. Escherichia

Q. The recombinant DNA technological process have made immense impact in the area of healthcare. How Eli Lilly produced Insulin ?

Q. Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
I. Synthesis of the gene (DNA) for human insulin artificially.
II. Culturing of recombinant E. coli in bacteria.
III. Purification of humulin.
IV. Insertion of human insulin gene in plasmid DNA.
V. Introduction of recombinant plasmid into E. coli.
VI. Extraction of recombinant gene product from E. coli.

1. II, I, IV, Ill, V, VI
2. I, III, V, VI, II, IV
3. I, V, IV, II , VI, III
4. I, IV, V, II , VI, III

Q. How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce an insulin?