DNA Microarray

Q. Which of the following is used as a probe in DNA fingerprinting?

1. Double stranded DNA
2. Double stranded RNA
3. Single stranded radioactive RNA
4. Single stranded radioactive DNA

Q. A DNA Microarray or ‘gene-chip’ consists of:

1. An RNA sample attached to the microarray surface and fluorescent labelled RNA probes
2. A single-stranded DNA probe attached to the microarray surface and fluorescent labelled single-stranded cDNA samples
3. An RNA probe attached to the microarray surface and fluorescent labelled DNA samples
4. A single-stranded DNA sample attached to the microarray surface and fluorescent labelled RNA probes

Q. Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given:
A. Electrophoresis of DNA fragments
B. Hybridization with DNA probe
C. Digestion of DNA by restriction endonucleases
D. Autoradiography
E. Blotting of DNA fragments nitrocellulose membrane

1. C – A – B – E – D
2. C – A – E – B – D
3. A – E – C – B – D
4. A – C – E – D – B

Q. Which of the following is used as a probe in DNA fingerprinting?

1. Double stranded DNA
2. Double stranded RNA
3. Single stranded radioactive RNA
4. Single stranded radioactive DNA

Q. The probe in a DNA microarray is ______.

1. A specified DNA molecule
2. Fixed to the glass slide
3. Single-stranded
4. All of the above

Q. The very first method used for DNA fingerprinting is

1. PCR analysis
2. RFLP analysis
3. STR analysis
4. AmpFLP

Q.

Probes, used in DNA fingerprinting, are initially.

1. Single stranded RNA

2. Minisatellite

3. 19 base long oligonucleotide

4. All of the above

Q. what is a probe in dna fingerprinting?

Q. DNA synthesis can be measured by estimating incorporation of radiolabeled

1. Uracil.
2. Ribose sugar.
3. Thymidine.
4. Adenine.

Q. The probe in a DNA microarray is ______.

1. A specified DNA molecule
2. Fixed to the glass slide
3. Single-stranded
4. All of the above