Genetic Material

Q.

Why salt is used in DNA extraction?

Q.

In recombinant DNA technology, antibiotics are used

1. As selectable markers

2. To keep media infection-free

3. As sequence where replication starts

4. To select the healthy vector

Q. The coding strand of DNA is :
5'-AATTCAAATTAGG-3'
What is the sequence of mRNA?

1. 3'-TTAAGTTTAATCC-5'
2. 5'-AAUUCAAAUUAGG-3'
3. 3'-AAUUCAAAUUAGG-5'
4. 5'-TTAAGTTTAATCC-3'

Q.

Why is chargaffs rule important to DNA?

Q.

If the distance between two consecutive base pairs is 0.34 Nm and the total number of base pairs of a DNA double helix in a typical mammalian cell Is 6.6 × 10⁹ Dp then the length of the DNA is approximately?

1. 2.2

2. 2.7

3. 2.0

4. 2.5

Q. The ability to transform a normal cell into a cancerous cell is found in

1. Any plasmid
2. Ti plasmid
3. Retrovirus
4. Both (b) and (c)

Q.

A specific recognition sequence identified by endonucleases to make cuts at specific within the DNA is:

1. Degenerate primer sequence

2. Okazaki sequences

3. Poly(A) tail sequences

4. Palindromic Nucleotide sequences

Q.

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?

1. Genetic code is specific

2. Genetic code is not ambiguous. Genetic code is nearly universal

3. Genetic code is redundant

4. Genetic code is nearly universal

Q.

While analyzing the DNA of an organism, a total number of 5386 nucleotides were found out of which the proportion of different bases were Adenine = 29%, Guanine = 17%, Cytosine = 32%, and Thymine = 17%. Considering Chargaff’s rule, it can be concluded that

It is single-stranded DNA.

It is double-stranded linear DNA.

No conclusion can be drawn.

It is a double-stranded circular DNA.

Q. A molecule that can act as a genetic material must fulfill the traits given, except

1. it should provide the scope for slow changes that are required for evolution
2. it should be able to express itself in the form of 'Mendelian characters'
3. it should be able to generate its replica
4. it should be unstable structurally and chemically

Q.

How is the length of the DNA defined?

Q.

Choose the correct option-

All of the following are part of an operon except?

1. An operator

2. An enhancer

3. Structural genes

4. A promoter

Q. Explain the mechanism of DNA replication.

Q.

What is positive regulation?

Q. Degeneracy of a genetic code is generally attributed to the

1. first member of a codon
2. second member of codon
3. entire codon
4. third member of a codon

Q.

What are transgenic bacteria? Illustrate using any one example.

Q.

How many types of nucleic acids are found in living systems?

Q. Which of the following is not a salient feature of genetic code?

1. Nearly universal
2. Ambiguous
3. Triplet
4. Degenerate

Q.

DNA was extracted from Bacterial Species. The Proportion of Cytosine was Found to be 30%, Then Calculate the Proportion of Purine.

Q. Western blotting is the technique for the detection of specific ______ in the sample.

1. protein
2. RNA
3. DNA
4. glycolipid

Q.

All living organisms are linked to one another because

(a) they have the common genetic material of the same type

(b) they share common genetic material but to varying degrees

(c) all have a common cellular organization

(d) All of the above

Q.

Which nitrogen base is absent in DNA?

1. Adenine

2. Guanine

3. Uracil

4. Cytosine

Q.

What is the width of a DNA molecule?

Q.

In vitro, clonal propagation in plants is characterized by

1. Electrophoresis and HPLC

2. Microscopy

3. Northern blotting

4. PCR and RAPD

Q. Which of the following implies that the genetic code is unambiguous?

1. Each codon codes for only one particular amino acid
2. Some amino acids can be coded by more than one codon
3. There are three codons which do not code for any amino acids
4. All of the above

Q. Which of the following cell types in the human body do not contain DNA?

1. White blood corpuscles
2. Liver cells
3. Nerve cells
4. Red blood corpuscles

Q. Which of the following can be the result of mutation?
A. Changes in phenotype
B. Changes in genotype
C. Variation in a population

1. A and B
2. A, B and C
3. B and C
4. B only

Q.

If the proportion of 5 methyl uracil in a ds-DNA is 7% then what will be the total proportion of monocyclic nitrogen bases in this DNA?

a)50% b)43% c)14%d)86%

And given:50%

Q. Flavr Savr Tomato has increased

1. Vigour
2. Flowering period
3. Productivity
4. Shelf life

Q. The height of a plant is under the control of polygenic inheritance. The genotype of a plant \(A_1 A_1 A_0 A_0\) is 56 cm and the height of plant \(a_1 a_1 a_0 a_0\) is 26 cm. What is the contribution, of each polygene?

1. 7.5cm
2. 15 cm
3. 30 cm
4. 8.5 cm