PCR in Diagnosis

Q. For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

1. Hybridization technique
2. Western Blotting technique
3. Southern Blotting technique
4. ELISA technique

Q. After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule?

1. 4
2. 32
3. 16
4. 8

Q.
Give full form of ELISA?

1. 
   Enzyme Linked Immuno Absorbant Assay
2. Enzyme Linking Immuno Assay
3. 
   Enzyme Live Implantations Assay
4. 
   Enzyme Live Immuno Absorbant

Q.

What are the disadvantages of ELISA?

Q.

What are the drawbacks of PCR?

Q.

What is the principle on which ELISA is based ?

Q.

What is the ELISA test used for?

Q. Automated-DNA sequencers are based on method developed by

1. Erwin Chargaff
2. Maurice Wilkins
3. Francis Crick
4. Frederick Sanger

Q.

A certain patient is suspected to be suffering from acquired immunodeficiency syndrome. Which diagnostic technique will you recommend for its detection?

1. MRI

2. ELISA

3. Ultrasound

4. WIDAL

Q. Which of the following is based upon the principle of antigen-antibody interaction?

1. PCR
2. ELISA
3. rDNA technology
4. Northern blotting

Q. The sensitivity of DNA fingerprinting technique has been _____ by the use of PCR.

1. increased
2. decreased
3. moderated
4. kept constant

Q. Which of the following cells act as initial HIV factories in the body?

1. T lymphocytes
   
2. Neutrophils
3. Erythrocytes
4. Macrophages

Q.

What are the advantages of ELISA?

Q.

What Are Touchdown PCR and Colony PCR?

Q. Which of the following is not required in the preparation of a recombinant DNA molecule?

1. Restriction endonuclease
2. DNA ligase
3. E. coli
4. DNA fragments

Q.

What is ELISA and its types?

Q. Why does the promoter gene decides the coding strand and temp3 strand?

Q. Transcription requires
A. DNA template
B. NTPs (nucleoside triphosphates)
C. RNA polymerase
D. Sigma & Rho factors

1. A, C
2. A, B, C, D
3. B, C, D
4. A, B, C

Q. Ligase is an enzyme required for

1. Breaking of DNA fragments
2. Joining DNA fragments
3. Proof reading
4. Renaturation of DNA

Q.

How Replication of HIV takes place?

Q.

Explain Hot start PCR.

Q.

Does DNA polymerase need a primer?

Q. Which of the following are the methods that can be included while testing for the presence of viral pathogens in the body at an early stage?
1. Urine analysis
2. ELISA
3. Autoradiography
4. Polymerase Chain Reaction (PCR)

1. 1, 2 and 3
2. 2, 3 and 4
3. 1, 3 and 4
4. 1, 2, 3, and 4

Q.

how does CH3 group in thymine facilitated its presence in DNA (or) why is thymine present in DNA instead of uracil.

Q. Which of the following statements is/are correct?
I. Recombinant DNA technology requires both nucleases and ligases.
II. Ligases produce nicks in the recombinant DNA molecule.
III. Nucleases produce the nick in the DNA molecule.

1. I, II
2. I, II and III
3. I and III
4. II and III

Q.

According to the definition of DNA fingerprinting, it is the determination of nucleotide sequences of certain areas of DNA which are unique to every individual. How do we know which part of the DNA is unique to everyone?

Q. AIDS can be detected by RT-PCR at an early stage as

1. it detects the antibodies produced against the virus
2. it amplifies even a single copy of RNA to produce millions of copies of cDNA
3. it avoids contamination of samples with other DNA
4. all of these

Q.

AIDS can spread from an infected person to another person through

1. sharing food

2. blood transfusion

3. sharing comb

4. a mosquito bite

Q.

What are the steps through which the process of immunoprecipitation is carried out?

Q.

What approaches can be employed for virus isolation?