Plasmids

Q.

Question 28

What are plasmids? Describe their role in bacteria.

Q. Which of the following is not a desirable feature of a cloning vector?

Q.

What are the three types of genetic recombination in bacteria?

Q.

One of the key factors, which makes plasmid the vector in genetic engineering is that

1. It is resistant to restriction enzymes.

2. It has the ability to carry a foreign gene.

3. It is resistant to antibiotics.

4. It has the ability to cause infection in the host.

Q.

How do you know if your transformation was successful?

Q. What are Episomes ???

Q. Autonomously replicating circular extrachromosomal DNA found in prokaryotic cell is called

1. satellite DNA
2. plasmid
3. recombinant DNA
4. nucleoid

Q. Plasmids are extra chromosomal circular DNA molecules

1. which have their own point of replication and can replicate independently
2. which have their own point of replicant but cannot replicate independently
3. none of the above
4. which do not have their own point of replication and cannot replicate independent of bacterial chromosomal DNA

Q.

How are plasmids classified?

Q. Some bacteria are able to grow in Streptomycin containing medium due to

1. Genetic drift
2. Reproductive isolation
3. Induced mutation
4. Natural selection

Q. What is true for plasmid ?

1. Plasmids are widely used in gene transfer
2. These are found in virus
3. Plasmid contain gene for vital activities
4. These are main part of chromosome

Q.

Some bacteria are able to grow in Penicillin containing medium due to

1. Genetic Drift

2. Reproductive isolation

3. Natural selection

4. Induced mutation

Q. In recombinant DNA technique the term vector refers to

1. plasmids that can transfer foreign DNA into a living cell
2. cosmids that can cut DNA at specific base sequence
3. plasmids that can join difference DNA fragments
4. cosmids that can degrade harmful proteins

Q.

How many linkage groups are present in bacteria?

Q. What are transgenic bacteria? Illustrate using any one example.

Q.

How many linkage groups are there in bacteria E Coli?

Q. Which of the following statement about plasmids is true?

1. Plasmid DNA is like phage DNA and needs the bacterial genome for replication
2. Each bacterial cell can have only one plasmid
3. Conjugation is one of the natural process through which plasmids can be transferred from one bacteria to another
4. All plasmids confer antibiotic resistance to the host bacteria

Q. What is true for plasmid ?

1. These are main part of chromosome
2. Plasmids are widely used in gene transfer
3. These are found in virus
4. Plasmid contain gene for vital activities

Q.

Question 35

The genomic content of the nucleus is constant for a given species where as the extrachromosomal DNA is found to be variable among the members of a population. Explain.

Q. Plasmids are ____

1. Extra chromosomal DNA which can self replicate
2. DNA carrying genetic sequence, without expressing it
3. integrated within host DNA without replication ability
4. none of these

Q. Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of -galactosidase enzyme and the colonies do not produce any colour.

1. Both statements 1 and 2 are correct
2. Statement 1 is correct but statement 2 is incorrect
3. Statement 1 is incorrect but statement 2 is correct
4. Both statements 1 and 2 are incorrect

Q. In recombinant DNA technique the term vector refers to

1. cosmids that can cut DNA at specific base sequence
2. plasmids that can join difference DNA fragments
3. plasmids that can transfer foreign DNA into a living cell
4. cosmids that can degrade harmful proteins

Q. Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid

Q.

The ultimate proof for the DNA as a genetic material came from the experiments of

1. Hershey and Chase
2. Avery, Macleod and McCarty
3. G. Mendel
4. Griffith

Q. Which of the following is a plasmid?

1. pBR 322
2. Bam H I
3. Sal I
4. Eco R I

Q. In bacteria, plasmid is:

1. Extra chromosomal material
2. Main DNA
3. Non functional DNA
4. Repetitive gene

Q. Drosophila melanogastor has 8 chromosomes in it's somatic cells. The number of possible linkage groups is

1. 5
2. 4
3. 8
4. 2

Q. Karyogamy is?

1. Fusion of two germ cells
2. Fusion of two gametic nuclei
3. Fusion of a somatic cell and a reproductive cell
4. Fusion of two somatic cells

Q. Have we discussed about two types of heterochromatin?

Q. Many bacteria have small circular DNA outside the genomic DNA. This extranuclear DNA

1. a and b
2. b and c
3. c and d
4. a and d