Process of DNA Replication

Q. Methyl guanosine triphopshate is added to 5' end of hn-RNA in a process of

1. tailing
2. splicing
3. capping
4. None of the above

Q. DNA replication in bacteria occurs :

1. Within nucleolus
2. During S phase
3. Prior to fission
4. Just before transcription

Q.

Where does replication of DNA occur in eukaryotes?

Q. Which of the following statements is/are CORRECT regarding transcription?

1. Genetic information is copied from RNA to DNA.
2. Both the DNA strands of the double helix serve as templates for transcription.
3. Only a segment of the template strand gets copied at a time.
4. Transcription takes place in the nucleus of the cell.

Q.

What is the end result of DNA replication?

Q. Explain sexual cycle of Fungi.

Q.

What is the function of primers in PCR reaction?

Q. Okazaki fragments are joined by

1. DNA polymerase
2. DNA ligase
3. DNA polymerase II
4. DNA polymerase I

Q. Assertion: : Insertion of recombinant DNA within the coding sequence of beta-galactosidase results in colourless colonies.

Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.

1. If both assertion and reason are true and reason is the correct explanation of assertion
2. If both assertion and reason are true but reason is not the correct explanation of assertion
3. If assertion is true but reason is false
4. If both assertion and reason are false

Q.

Is ligase used on the leading strand?

Q.

What is the role of ORI in pBR322?

Q. When the DNA replication starts, the

1. Leading strand produces Okazaki fragment
2. Hydrogen bonds between the nucleotides of two strand break
3. Phosphodiester bond between the adjacent nucleotide break
4. Bonds between the nitrogen base and deoxyribose sugar break

Q.

What happens if primase is not present?

Q.

Which of the following properites of DNA polymerise necessitates the requirement of RNA primer in DNA replication?

1. It gets denatured at high temperatures.

2. It can add a nucleotide only to a 5' end

3. It can add a nucleotide only to a 3' end

4. None of the above

Q. When the DNA replication starts, the

1. Leading strand produces Okazaki fragment
2. Hydrogen bonds between the nucleotides of two strand break
3. Phosphodiester bond between the adjacent nucleotide break
4. Bonds between the nitrogen base and deoxyribose sugar break

Q. DNA acts as a template for synthesis of

1. DNA
2. RNA
3. Both DNA and RNA
4. Protein

Q. New strand formation on a DNA template can be initiated only by

1. DNA polymerase
2. DNA polymerase III
3. RNA primer
4. DNA primer

Q. Sir/Mam, during DNA replication, what is tge role of a rna primer.Also why there is rna primer not dna primer?

Q. Formation of RNA over the template of DNA is

1. Replication
2. Translation
3. Transversion
4. Transcription

Q. The elongation of the DNA strand during DNA synthesis

1. progresses away from the replication fork
2. depends on the action of RNA polymerase
3. depends on the action of DNA polymerase
4. occurs in the 3’ to 5’ direction

Q.

Match the following enzymes of DNA replication with their respective functions:
$\begin{array}{cc}p.DNApolymerase& i.HelpsunziptheDNAdoublehelix\\ q.DNAligase& ii.SealsnicksbetweenOkazakifragments\\ r.DNAhelicase& iii.LaysRNAprimerstowhichpolymerasecanaddnucleotides\\ s.Primase& iv.Addsnucleotidestothefree{3}^{\prime }endofRNAprimers\end{array}$

1. p - i, q - ii, r - iii, s - iv

2. p - iii, q - i, r - ii, s - iv

3. p - iv, q - ii, r - i, s - iii

4. p - ii, q - iii, r - iv, s - i

Q. Which one of the following is an accurate statement regarding chromosome replication?

1. It occurs during G1 of the cell cycle
2. Inactive genes are replicated first.
3. It starts with the synthesis of Okazaki fragments.
4. It is semi-conservative

Q.

How are replication errors corrected?

Q.

Okazaki fragments are formed during:

1. Transduction

2. Transcription

3. Replication

4. Translation

Q. The elongation of the DNA strand during DNA synthesis

1. progresses away from the replication fork
2. occurs in the 3’ to 5’ direction
3. depends on the action of RNA polymerase
4. depends on the action of DNA polymerase

Q.

How does DNA ligation work?

Q. cDNA is formed by

1. RNA dependent DNA polymerase
2. DNA ligase
3. DNA dependent DNA polymerase
4. DNA dependent RNA polymerase

Q. DNA can be formed by

1. Transaminase
2. Lyases
3. RNA dependent DNA polymerase
4. All of the above

Q. Enzyme required during DNA replication is

1. DNA polymerase
2. DNA ligase
3. Both A and B
4. Sigma factor

Q.

What is a methylation-specific PCR?