Selection of Transformed Cell

Q.

A foreign DNA is inserted within the Pst I site of the $Am{p}^R$ gene in vector pBR322. Host bacteria were transformed with the engineered plasmid. Choose incorrect statement(s) from the following:
A. Recombinants will lose ampicillin resistance.
B. Recombinants will grow on a medium containing ampicillin but not on that containing tetracycline.
C. Non-recombinant transformed cells will grow on a medium containing both the antibiotics.
D. Recombinants will grow on a medium containing tetracycline but not on that containing ampicillin.

Q. In blue/white screening, the transformed cells with rDNA will exhibit

1. blue colour
2. white colour
3. blue and white colour
4. no colonies

Q. Insertional inactivation is related to:

1. Micro injection
2. Gene gun
3. Gel electrophoresis
4. Selection of recombinants

Q. Insertional inactivation is related to:

1. Micro injection
2. Gene gun
3. Gel electrophoresis
4. Selection of recombinants

Q. Plasmids are self replicating, extrachromosomal segments of single strand.

1. true
2. false

Q. Assertion :The cloning vector is required to have very few, preferably single recognition commonly used used restriction enzymes. Reason: Presence of more than one recognition site within a cloning vector will generate which will complicate the process of gene cloning.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. In microscopy, a nucleus is normally stained using

1. Neutral red
2. Janus green B
3. Eosin
4. Hematoxylin

Q. Which gene codes for the proteins involved in the regulation of vector copy number?

1. ori
   
2. ampR
3. rop
4. tetR
   

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

1. The eukaryotic DNA and plasmid DNA will have the same sequence
2. The eukaryotic DNA and plasmid DNA will have complementary sequences
3. The eukaryotic DNA can join with any plasmid DNA, regardless of sequence
4. The plasmid DNA can join with any eukaryotic DNA, regardless of sequence
5. The plasmid DNA and the eukaryotic DNA cannot join together due to differences in the structure of their DNA molecules

Q. Toadstool mushrooms are poisonous because they inhibit

1. DNA replication
2. Transcription
3. Synthesis of peptides at ribosomal level
4. All of the above

Q. _________ is produced by virus infected cell that can protect non-infected cells from further viral infection.

1. Serotonin
2. Colostrum
3. Interferon
4. Histamine

Q. When a dicot plant is infected by Agrobacterium tumefaciens, the T-DNA in Ti-plasmid induces the plant to produce?

1. Growth hormones like auxins and cytokinins
2. Growth inhibitors like absciscic acid
3. Fatty acids
4. Leghaemoglobin

Q. Some R-plasmids carry genes for resistance to many antibiotics. This occurs naturally due to :

1. Conjugation
2. Transduction
3. Transformation
4. Transgenesis

Q.

a recombinant vector with a gene of interest inserted within a gene of alpha-galactosidase enzyme is introduced into a bacterium. explain the method that would help in the selection of recombinant colonies frm non recombinant ones? why this method of selection is reffered as Insertional Inactivation?

Q. What is the role of tetracyclin resistant gene in plasmid vector?

1. Improves the stability of the plasmid
2. Increases the ability of plasmid to accomodate gene of interest
3. Gives tertacyclin immunity against accidental antibiotic presence
4. Helps to identify the cells that are transformed

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except

1. Each restriction enzyme recognizes a specific sequence of bases on the DNA molecule
2. Each restriction enzyme cuts at random locations along the DNA molecule
3. Most restriction enzymes are named after the bacterial organism from which they were first isolated
4. Restriction enzymes protect their bacterial host against intruding foreign DNA from viruses or other bacterial cells
5. When a particular sequence of DNA is digested with a specific restriction enzyme, the resulting set of restriction fragments will usually be the same

Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media - one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

1. will grow on both tetracycline containing and ampicillin containing media
2. will not grow on either tetracycline containing or ampicillin containing media
3. will not grow on tetracycline, but not on ampicillin-containing media
4. will grow on ampicillin but not on tetrecycline-containing media

Q. The distribution of two or more specific molecules within a cell can be studied by using

1. Dark field microscope
2. Flourescent microscope
3. Interference contrast microscope
4. Bright field microscope
5. Phase contrast microscope

Q. During genetic mutation, mutagen adds a nucleotide to a protein-coding sequence of DNA and creates an early stop codon. Which of the following will occur?

1. The primary structure of the protein will be unaffected.
2. The secondary structure of the protein will be unchanged.
3. The protein will not be translated at all.
4. The protein will be complete but function abnormally.
5. The protein will be synthesized normally up to the mutation.

Q. State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.

Q. Barley endosperm test is a bioassay for

1. Auxin
2. Ethylene
3. Morpbactin
4. Gibberellin

Q. One of the following is a wrong match.

1. Micropropagation - In vitro production of plants in large numbers
2. Callus - Unorganised mass of cells produced in tissue culture
3. Somatic hybridization - Fusion of two diverse cells
4. Vector DNA - Site for tRNA synthesis

Q.

State what happens when a gene is inserted in Sal I site of pBR322 plasmid.

Q. Sex factor in bacteria is

1. F- pilus
2. RNA
3. Chromosomal replicon
4. None of above

Q. Which of the following parts of the plasmid can help in selection of transformed organism?

1. Replicon
2. Ori site
3. Antibiotic resistance gene
4. Gene of interest

Q. Where do restriction enzymes come from?

Q. Mention the type of host cells suitable for the gene guns to introduce an alien DNA.

Q. Which of the following help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants?

1. Selectable markers
2. Structural genes
3. Recognition sites
4. Ori sites

Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

1. Will not grow on either tetracycline containing or ampicillin containing media
2. Will grow on both tetracycline containing and ampicillin containing media
3. Will grow on ampicillin but not on tetracycline containing medium
4. Will grow on tetracycline but not on ampicillin containing medium

Q. What is the role of tetracyclin resistant gene in plasmid vector?

1. Improves the stability of the plasmid
2. Increases the ability of plasmid to accomodate gene of interest
3. Gives tertacyclin immunity against accidental antibiotic presence
4. Helps to identify the cells that are transformed