Ti Plasmid
Trending Questions
Q. Which of the following statements regarding Baculoviruses are correct?
- Baculoviruses are pathogens that attack insects and other arthropods
- Most of Baculoviruses are biocontrol agents belonging to the genus Nucleopolyhedrovirus
- Baculoviruses do not harm non-target insects
- All of the above
Q. What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Q. In the agrobacterium mediated gene transfer using leaf disc method, what is the significance of cutting the leaf into discs?
- To make the leaf round shape
- To induce induce dedifferentiation in leaf cells
- To increase surface area for transfers
- To use both surfaces of the leaf
Q. The tumor inducing (Ti) plasmid has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants because Ti plasmid has been modified by :
- Deleting tumor forming genes
- Adding tumor forming genes
- Deleting endonuclease
- Adding genes resistant to endonucleases
Q. Mutations in plant cells can be induced by
- Zeatin
- Kinetin
- Infrared rays
- Gamma rays
Q. The proteins encoded by Cry I Ac and Cry II Ab genes control.
- Boll worms
- Pin worms
- Book worms
- Nematodes
Q. Transgenes are transferred to plants using Ti plasmid present in:
- Salmonella typhimurium
- Agrobacterium tumefaciens
- Bacillus thuringiensis
- Pseudomonas fluorescens
Q. Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?
- BAC, cosmid, phage, plasmid, YAC
- YAC, BAC, cosmid, phage, plasmid
- Plasmid, Cosmid, phage, BAC, YAC
- Plasmid, phage, cosmid, BAC, YAC
Q. The bacteria associated with plant genetic engineering are
- Salmonella and Pseudomonas
- Salmonella typhimurium and Agrobacterium tumefaciens
- Bacillus thuringiensis and Pseudomonas fluorescens
- Rhizobium leguminosarum and Azotobacter agilis
Q. Cry genes or Bt genes are obtained from ?
- Cotton pest
- Tobacco pest
- Bacillus thuringiensis
- E.coli
Q. Observe the diagram and answer the following questions
AOOTING MEDIUM + KANAMYCIN + KAAAMYCIN + CARBENICILLIN CARBENICILLIN SHOOTS REGENERLATE IN 3-4 WEEKS
AOOTING MEDIUM + KANAMYCIN + KAAAMYCIN + CARBENICILLIN CARBENICILLIN SHOOTS REGENERLATE IN 3-4 WEEKS
Q. Plasmids are vectors for gene cloning because they
- Self replicate in bacterial cells
- Replicate freely outside bacterial cells
- Can be multiplied in culture
- Can be multiplied in laboratories using enzymes
Q. The Ti-plasmid is used for making transgenic plants. This plasmid is found in
- Agrobacterium
- Yeast as a 2µm plasmid
- Azotobacter
- Rhizobium of the roots of leguminous plants
Q. Transgenes are transferred to plants using Ti plasmid present in
- Salmonella typhimurium
- Bacillus thuringiensis
- Agrobacterium tumefaciens
- Pseudomonas fluorescens
Q. In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?
- To make the leaf round shape
- To use both surfaces of the leaf
- To induce induce dedifferentiation in leaf cells
- To increase surface area for transfers
Q. The bacteria associated with plant genetic engineering are:
- Azotobacter agilis
- Agrobacterium tumefaciens
- Bacillus thuringiensis
- Rhizobium leguminosarum
Q. 'Cry' gene is obtained from
- Agrobacterium tumefaciens
- Bacillus thuringiensis
- Rhizobium leguminosarum
- Rhizobium phaseoli
Q. Which bacteria produces Bt toxin?
Q. In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?
- To make the leaf round shape
- To induce induce dedifferentiation in leaf cells
- To increase surface area for transfers
- To use both surfaces of the leaf
Q. The process in which (genetic material is carried by some external agency) viruses are involved in sexual reproduction of bacteria is called
- Transcription
- Transduction
- Transformation
- Translation
Q. Wild Ti plasmid is able induce tumors in plants due to presence of
- Cancer genes
- Phytohormone coding genes
- Kanamycin resistance gene
- Cry genes
Q. Which one of the following is used as vector for cloning genes into higher organisms?
- Retrovirus
- Baculovirus
- Salmonella typhimurium
- Rhizopus-nigricans
Q. Which one can be used as vector for transfer of genes?
- BAC
- Plasmid
- YAC
- All of the above
Q. Which among the following is a tumour inducing plasmid widely used in the production of transgenic plants?
- Escherichia coli
- Bacillus thuringiensis
- Staphylococcus aureus
- Agrobacterium tumefaciens
Q. Which of the following modifications are made to make a “tamed” Ti plasmid which can transfer cry genes to a plant’s genome ?
- Removal of kanamycin resistance gene and adding phytohormone genes
- Removal of phytohormone gene and adding kanamycin resistance gene
- Removal of both kanamycin resistance gene and phytohormone genes
- Adding both phytohormone and kanamycin resistance gene
Q. Bacillus thuringiensis strains have been used for
- Biomineralization processes
- Bioinsecticidal application
- Biofertilizer application
- Biometallurgical techniques
Q. Transfer of genetic information through transduction involves
- Conjugation
- Bacteriophage released from the donor cell
- Another bacterium
- Physical contact between donor and recipient strains
Q. Transfer of genetic material with the help of a virus is called
- Transduction
- Transference
- Transformation
- Transcription
Q. The introduction of T-DNA into plants involves:
Q. In which step the gene of interest gets incorporated in plant genome?
- Growing the discs with shoot inducing medium
- Growing the leaf discs with agrobacterium
- Cutting the leaf discs
- Growing the discs with root inducing medium