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A and B are t...

Question

A and B are the two different cloning vectors in two different bacterial colonies cultued in chromogenic substrate. bacterial colonies with cloning vector A was colourless, whereas those with B were blue coloured. explain giving reasons the cause of the difference in colour that appeared

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Solution

Dear Student,

The selection of recombinants is carried out by insertional inactivation.
Insertional inactivation is the loss of function of a gene such as α-galactosidase gene in plasmid due to insertion of foreign DNA/transgene within it.
Because of the inactivation of the gene α-galactosidase, the non-recombinants (bacterial colonies with cloning vector B) will produce blue-coloured colonies while the recombinants (bacterial colonies with cloning vector A) will produce colourless colonies.

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