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A certain purified DNA was cut with two restriction endonucleases E1 and E2. The results were obtained from agarose gel electrophoresis

Sample cut with E1 alone: two bands of size 35 kb and 15 kb

Sample cut with E2 alone: two bands of size 40 kb and 10 kb

Sample cut with E1 and E2 simultaneously: three bands of size 35 kb, 10kb and 5 kb

From this data it can be inferred that the DNA has


A
2 sites for E1 and 1 site for E2
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B
1 site for E1 and 2 site for E2
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C
1 site each for E1 and E2
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D
3 sites for E1 and 1 site for E2
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Solution

The correct option is C 1 site each for E1 and E2

The total weight of DNA fragment is 50 kb

Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at 40kb.


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