1
Question
A certain purified DNA was cut with two restriction endonucleases
E1 and E2. The results were obtained from agarose gel electrophoresis
Sample cut with E1 alone: two bands of
size 35 kb and 15 kb
Sample cut with E2 alone: two bands of
size 40 kb and 10 kb
Sample cut with E1 and E2
simultaneously: three bands of size 35 kb, 10kb and 5 kb
From this data it can
be inferred that the DNA has
A certain purified DNA was cut with two restriction endonucleases E1 and E2. The results were obtained from agarose gel electrophoresis
Sample cut with E1 alone: two bands of size 35 kb and 15 kb
Sample cut with E2 alone: two bands of size 40 kb and 10 kb
Sample cut with E1 and E2 simultaneously: three bands of size 35 kb, 10kb and 5 kb
From this data it can be inferred that the DNA has
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Solution
The correct option is C 1 site each for E1 and E2
The total weight of DNA fragment is 50
kb
Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at
40kb.
The total weight of DNA fragment is 50 kb
Enzyme E1 cuts at 35 kb and enzyme E2 cuts DNA at 40kb.
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