A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoR- and one for Hind- All of them are equidistant from each other- Upon incubation with both the restriction enzymes bands appear in the gel electrophoresis-

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Standard XII

Biology

Gel Electrophoresis

A circular do...

Question

A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoRⅠ and one for HindⅢ. All of them are equidistant from each other. Upon incubation with both the restriction enzymes _____ band(s) appear in the gel electrophoresis.

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Solution

The correct option is C 1
Since the DNA sample incubated was circular having three restriction sites equidistant from each other, three fragments of equal size are created by restriction enzyme action. Upon gel electrophoresis, a single band will appear at 15 kb.

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Q. Two DNA samples A and B, contain equal number of nucleotides and both of them have one restriction site for EcoRⅠ and Hind Ⅲ. On complete digestion with both the restriction enzymes, A forms 3 bands and B forms 2 bands on gel electrophoresis.
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Q. Which of the following sequences along a double stranded DNA molecule may be recognized as a cutting site for a particular restriction enzyme ?

A certain purified DNA was cut with two restriction endonucleases E1 and E2. The results were obtained from agarose gel electrophoresis

Sample cut with E1 alone: two bands of size 35 kb and 15 kb

Sample cut with E2 alone: two bands of size 40 kb and 10 kb

Sample cut with E1 and E2 simultaneously: three bands of size 35 kb, 10kb and 5 kb

From this data it can be inferred that the DNA has

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A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoRⅠ and one for HindⅢ. All of them are equidistant from each other. Upon incubation with both the restriction enzymes _____ band(s) appear in the gel electrophoresis.

The correct option is C 1Since the DNA sample incubated was circular having three restriction sites equidistant from each other, three fragments of equal size are created by restriction enzyme action. Upon gel electrophoresis, a single band will appear at 15 kb.

The correct option is C 1
Since the DNA sample incubated was circular having three restriction sites equidistant from each other, three fragments of equal size are created by restriction enzyme action. Upon gel electrophoresis, a single band will appear at 15 kb.