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Question
A double-stranded DNA sample when separately incubated with EcoRⅠ and Hind Ⅱ, produces a single band at 10.2 kb in agarose gel electrophoresis.
Based on the above information which of the following statements is correct?
A double-stranded DNA sample when separately incubated with EcoRⅠ and Hind Ⅱ, produces a single band at 10.2 kb in agarose gel electrophoresis.
Based on the above information which of the following statements is correct?
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Solution
The correct option is C It is a double-stranded circular DNA
When DNA is separately incubated with EcoRⅠ and Hind Ⅱ, it produces a single band in gel electrophoresis at 10.2 kb. The following inference is made based on the above information:
The DNA contains a single restriction site for both of EcoRI and Hind II restriction endonucleases.
Since a single band appears at 10.2 kb, it means the initial circular DNA after the cut becomes linear.
So the above sample has double-stranded circular DNA.
When DNA is separately incubated with EcoRⅠ and Hind Ⅱ, it produces a single band in gel electrophoresis at 10.2 kb. The following inference is made based on the above information:
The DNA contains a single restriction site for both of EcoRI and Hind II restriction endonucleases.
Since a single band appears at 10.2 kb, it means the initial circular DNA after the cut becomes linear.
So the above sample has double-stranded circular DNA.
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