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Ligase

A foreign DNA...

Question

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form recombinant plasmid using

A

Ligase

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B

Eco RI

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C

Taq polymerase

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D

Polymerase II

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Solution

The correct option is A Ligase
So the correct option is A.

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Similar questions

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Arrange the following steps of formation of recombinant DNA in the correct sequence.
I. Complementary sticky ends are produced on the foreign DNA and the plasmid.
II. Foreign DNA and plasmids are mixed together
III. DNA ligase is used to seal the nicks in the sugar–phosphate backbone.
IV. Cut the foreign DNA (containing the DNA fragment of interest) and the plasmid with the same restriction enzyme.

Q. A piece of foreign DNA was inserted into a plasmid with an antibiotic resistance gene and lactose Z gene. The plasmid DNA was cut with a restriction enzyme, which splits the lactose Z gene and opens the circle. The foreign DNA was next inserted into the open restriction site of the plasmid. When the recombinant plasmid was introduced into bacterial cells and grown in the presence of antibiotic, some of the colonies turned blue in the presence of gene. The blue colonies contained

Q. Identify the correct sequence of steps in the construction of recombinant DNA.

(1)

use restriction enzymes

(2)

(3)

remove plasmid from parent bacterium

(4)

introduce plasmid into new host bacterium.

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

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