Question

A mixture containing DNA fragments a, b, c and d with molecular weights of a+b = c, a > b and d > c, was subjected to agarose gel electrophoresis. The movement of these fragments from cathode to anode sides of the gel would be

• A. Smaller molecules move faster
• B. Both smaller and larger will move at same pace
• C. Larger will move faster
• D. None of these

Answer 1

The correct option is A Smaller molecules move faster

• In Agarose gel electrophoresis we separate macromolecules like DNA, RNA and protein on the basis of their molecular size and charge.
• In this Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel.
• DNA migrate towards anode.