CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon


Question


(a) Name the enzyme P and Q. 
(b) What is the role of enzyme P?
(c) Can two different enzymes be used to cut foreign DNA and vector DNA? Give reasons. [1+1+1] 
 


Solution

(a) Enzyme P is a restriction enzyme and enzyme Q is a DNA ligase. [1] 

(b) Restriction enzyme (P) cuts both the foreign DNA and vector DNA at specific sites. [1] 

(c) No, two different enzymes cannot be used to cut foreign DNA and vector DNA because the resultant DNA fragments after cutting should have the same kind of ‘sticky-ends’ that can be joined together (end-to-end) using DNA ligases. These sticky ends can be obtained only if both are cut by the same restriction enzyme.[1] 

flag
 Suggest corrections
thumbs-up
 
0 Upvotes


Similar questions
QuestionImage
QuestionImage
View More...



footer-image