Question

A person who is detected positive to Elisa test. The next best test to establish the disease is

• A. Western blot
• B. Southern blot
• C. PCR
• D. Both A and C

Answer 1

The correct option is A Western blot

• Western blotting is the immunoblotting because the antibody is used and antigen-antibody interaction helps to identify the target protein from the complex mixture of protein.
• It uses gel electrophoresis to separate target protein on the basis of length of the polypeptide then the protein from the electrophoretic gel is transferred to nitrocellulose membrane where they are stained with antibodies specific to the target protein.
• A positive ELISA test is always followed by a western blot test. A positive Western blot confirms the infection, while a negative western blot means the ELISA test was a false positive test.
  Thus, the correct answer is option A.