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Question
A plasmid isolated from Salmonella is introduced into E.coli.
The plasmid replicates within E.coli by making use of which of the following enzymes?
A plasmid isolated from Salmonella is introduced into E.coli.
The plasmid replicates within E.coli by making use of which of the following enzymes?
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Solution
The correct option is D DNA polymerase
DNA replication refers to the process by which identical copies of DNA are synthesised within the cells.
Plasmids are extrachromosomal circular DNA molecules found in certain bacteria. They are capable of replicating autonomously due to the presence of an ori (origin of replication) site.
The plasmid isolated from Salmonella replicates within E.coli with the help of DNA polymerase.
DNA polymerase is an enzyme that synthesises the DNA molecules from nucleoside triphosphates, the molecular precursors of DNA.
Ligases are enzymes that are used to catalyse linking reactions by joining two large molecules by establishing a new chemical bond between them. For example, DNA ligase catalyses the joining of the 3′-OH to the 5′-phosphate, by forming a phosphodiester bond between two successive nucleotides.
Nucleases are a broad and diverse class of enzymes that hydrolyse the phosphodiester bonds of DNA and RNA.
Restriction enzymes are a type of nucleases that cleave DNA at or close to different recognition sequences known as restriction sites. These enzymes make a single incision on each of the two DNA strands and are also called restriction endonucleases.
DNA replication refers to the process by which identical copies of DNA are synthesised within the cells.
Plasmids are extrachromosomal circular DNA molecules found in certain bacteria. They are capable of replicating autonomously due to the presence of an ori (origin of replication) site.
The plasmid isolated from Salmonella replicates within E.coli with the help of DNA polymerase.
DNA polymerase is an enzyme that synthesises the DNA molecules from nucleoside triphosphates, the molecular precursors of DNA.
Ligases are enzymes that are used to catalyse linking reactions by joining two large molecules by establishing a new chemical bond between them. For example, DNA ligase catalyses the joining of the 3′-OH to the 5′-phosphate, by forming a phosphodiester bond between two successive nucleotides.
Nucleases are a broad and diverse class of enzymes that hydrolyse the phosphodiester bonds of DNA and RNA.
Restriction enzymes are a type of nucleases that cleave DNA at or close to different recognition sequences known as restriction sites. These enzymes make a single incision on each of the two DNA strands and are also called restriction endonucleases.
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