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Question

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

A
Electrophoresis
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B
Blotting
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C
Autoradiography
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D
PCR
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Solution

The correct option is D PCR
An analysis of chromosomal DNA using the Southern hybridization technique involves the three following steps:
  • Gel electrophoresis
  • Blotting
  • Autoradiograph
PCR or polymerase chain reaction is used to amplify a single copy or a few copies DNA of interest. This process is not employed during a Southern hybridization technique.

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