The correct option is A Mutation → Reproductive isolation → Natural selection → Evolution
The correct sequence is:
Mutation → Reproductive isolation → Natural selection → Evolution
A mutation is the permanent alteration of the nucleotide sequence of the genome of an organism which leads to formation of new species. The species with a difference in its genotype may not be able to interbreed with the original species, leading to reproductive isolation. Usually, mutations and variations occur in order to have a better adaptation to the environment and the organisms which are unable to adapt will not survive which indicates natural selection. This then leads to evolution.