Question

Arrange the various steps of DNA fingerprinting technique in the correct order.
(i) Separation of DNA fragments by electrophoresis.
(ii) Digestion of DNA by restriction endonucleases.
(iii) Hybridization using labelled VNTR probe.
(iv) Isolation of DNA.
(v) Detection of hybridized DNA fragments by auto radiography.
(vi) Transferring the separated DNA fragments to nitrocellulose membrane.

• A. $\left(iv\right)\to \left(ii\right)\to \left(i\right)\to \left(vi\right)\to \left(iii\right)\to \left(v\right)$
• B. $\left(iv\right)\to \left(i\right)\to \left(ii\right)\to \left(iii\right)\to \left(vi\right)\to \left(v\right)$
• C. $\left(ii\right)\to \left(i\right)\to \left(iv\right)\to \left(vi\right)\to \left(iii\right)\to \left(v\right)$
• D. $\left(iii\right)\to \left(v\right)\to \left(iv\right)\to \left(ii\right)\to \left(i\right)\to \left(vi\right)$

Answer 1

The correct option is A $\left(iv\right)\to \left(ii\right)\to \left(i\right)\to \left(vi\right)\to \left(iii\right)\to \left(v\right)$

Steps of DNA fingerprinting technique: Isolation of DNA $\to$ Digestion of DNA by restriction endonucleases $\to$ Separation of DNA fragments by electrophoresis $\to$ Transferring the separated DNA fragments to nitrocellulose membrane $\to$ Hybridization using labelled VNTR probe $\to$ Detection of hybridized DNA fragments by auto radiography.