Question

Study the following sequence and answer the questions:
5' ATATTACGAGCTATCGGA 3'
a. Is this a template strand or a coding strand?
b. What would be the sequence of mRNA strand transcribed from this strand?
c.How many amino acids would be incorporated using the above mRNA?
​d. With the help of a checker board, identify the amino acid specified by this strand.

Answer 1

(a). It is a template strand as it reads from 5' to 3'.
(b) The sequence of mRNA strand transcribed from this would be UAUAAUGCUCGAUAGCCU.
(c) There are 18 nucleotides in this mRNA strand. One amino acid is coded by 3 nucleotides. Hence, there will be 6 amino acids coded by this mRNA strand.
(d) Amino acids coded by this strand would be:

• UAU- Tyr
• AAU- Asn
• GCU- Ala
• CGA- Arg
• UAG- Stop Codon
• CCU- Pro