Can you
think and answer how a reporter enzyme can be used to monitor
transformation of host cells by foreign DNA in addition to a
selectable marker?
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
A reporter
gene can be used to monitor the transformation of host cells by
foreign DNA. They act as a selectable marker to determine whether
the host cell has taken up the foreign DNA or the foreign gene gets
expressed in the cell. The researchers place the reporter gene and
the foreign gene in the same DNA construct. Then, this combined DNA
construct is inserted in the cell. Here, the reporter gene is used as
a selectable marker to find out the successful uptake of genes of
interest (foreign genes). An example of reporter genes includes lac Z
gene, which encodes a green fluorescent protein in a jelly fish.
A reporter gene can be used to monitor the transformation of host cells by foreign DNA. They act as a selectable marker to determine whether the host cell has taken up the foreign DNA or the foreign gene gets expressed in the cell. The researchers place the reporter gene and the foreign gene in the same DNA construct. Then, this combined DNA construct is inserted in the cell. Here, the reporter gene is used as a selectable marker to find out the successful uptake of genes of interest (foreign genes). An example of reporter genes includes lac Z gene, which encodes a green fluorescent protein in a jelly fish.
