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Question

Choose the correct answers from the alternatives given.
What is the function of DNA ligase in recombinant technology?

A
Cut DNA into many fragments
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B
Carry DNA into a new cell
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C
Link together newly joined fragments of DNA
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D
Separate fragments of DNA by their length and electrical charges
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Solution

The correct option is C Link together newly joined fragments of DNA
DNA ligase joins the DNA strands together by catalyzing the formation of a phosphodiester bond between the 5' phosphate of one strand and 3'hydroxyl of one strand. They can join both DNA (DNA ligase) and RNA (an RNA ligase). It plays a role in repairing single-strands breaks in duplex DNA in a living organism but some forms may specifically repair double-strand breaks.
So, the correct option is 'Link together newly joined fragments of DNA'.

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