Question

DNA fragments generated by restriction endonucleases in a chemical reaction can be separated by

• A. Restriction mapping
• B. Centrifugation
• C. Polymerase chain reaction
• D. Agarose gel electrophoresis

Answer 1

The correct option is D Agarose gel electrophoresis

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by electrophoresis. Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is used to move molecules to be separated through a gel (agarose gel). Pores in the gel work like a sieve, allowing smaller molecules to move faster than larger molecules. The conditions used during electrophoresis can be adjusted to separate molecules in the desired size range.

So the correct answer is option D.