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Question

DNA helicase helps in

A
Opening supercoils of DNA
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B
Making DNA helix
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C
Opening two chains of DNA helix
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D
Making hydrogen bonds of nitrogenous bases
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Solution

The correct option is C Opening two chains of DNA helix
Helicases move along the double-stranded DNA and separate the strands by breaking hydrogen bonds at the expense of energy from ATP. DNA unwinding by helicases creates topological stress/supercoils in the helical DNA structure which is relieved by the action of topoisomerases. These enzymes transiently break both DNA strands of one chromosome thereby making the other chromosome to pass through the break and relieve the stress. DNA polymerase enzyme synthesizes DNA strands by adding deoxyribonucleotides to 3' end of the primer via phosphodiester bonds; it uses parental DNA as template. Hydrogen bonding between base pairs is facilitated by the presence of hydrogen bond donor (primary and secondary amine groups or hydroxyl groups) and complementary hydrogen bond acceptor (carbonyls and tertiary amines) in bases; it is not enzymatic process. The correctoption is C.

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