DNA segment in a neutral pH solution are negatively charged, there are two excess electrons per base pair if a 0.1 micron DNA fragment has a charge of −9.1×10−17C, how many basepairs are there along this fragment?
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Solution
Electron charge =−1.60217662×10−19Coulombs There are 2 electrons per base pair, and total charge of 0.1 microns of DNA is −9.1×10−17Coulombs. (−9.1×10−17)(−1.60217662×10−19)=568 As, 2 electrons make 1 base pair. 5682=284 base pairs.