Question

Are sticky ends created by Hindlll?

Answer 1

Hindlll

1. Hindlll is a restriction enzyme.
2. A restriction enzyme cleaves DNA into fragments at or near restriction sites within molecules.
3. When certain restriction enzymes digest the DNA, the ends of a DNA strand are left with one strand overhanging the other, forming a short single-stranded segment.
4. This overhang is known as "sticky ends" because it easily re-attaches to other similar ends.
5. The restriction enzyme HindIII produces sticky ends.
6. The recognition site is a palindrome with the letters AAGCTT in the sequence.
7. It cuts the DNA between the two AA.