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Does Restrict...

Question

Does Restriction exonuclease require palindromic sequence?

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Solution

Yes , restriction enzymes are restriction/palindromic site restricted

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Q. Assertion :Restriction endonuclease recognize short palindromic sequence and cut at specific sites. Reason: When a restriction endonuclease acts on palindrome, it cleaves both the strands of DNA molecule.

Q. Assertion :Restriction enzymes recognise palindromic sequences. Reason: Palindromic sequences read same in both directions of the two strands.

Q. (a) Why are restriction endonucleases so called ? (b) What is a palindromic nucleotide sequence ? How do restriction endonucleases act on palindromic sites to create sticky ends ?

Q. Which palindromic base sequence is cut at the middle by a restriction enzyme

Q. Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA. Explain.

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