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Question

During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

A
DNA ligase
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B
Restriction endonuclease
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C
Alkaline phosphatase
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D
Exonuclease
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Solution

The correct option is B DNA ligase
  • The process of cloning starts with the cleavage of foreign DNA by a restriction endonuclease enzyme.
  • The cleaved DNA is ligated to the plasmid vector for its transformation into the host cell.
  • The DNA is ligated by DNA ligase by the formation of phosphodiester bonds.

So, the correct answer is option A.

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