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Standard XII
Biology
Ligase
During gene c...
Question
During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is
A
DNA ligase
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B
Restriction endonuclease
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C
Alkaline phosphatase
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D
Exonuclease
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Solution
The correct option is
B
DNA ligase
The process of cloning starts with the cleavage of foreign DNA by a restriction endonuclease enzyme.
The cleaved DNA is ligated to the plasmid vector for its transformation into the host cell.
The DNA is ligated by DNA ligase by the formation of phosphodiester bonds.
So, the correct answer is option A.
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Similar questions
Q.
During gene cloning, the enzyme used to join and insert DNA within plasmid is
Q.
Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant
molecule?
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Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is
true about restriction enzymes except
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The vector plasmid and the gene of interest that have been cut with the same restriction enzyme creating sticky ends are joined by
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Q.
Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
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e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?
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Standard XII Biology
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