Question

During translation initiation in prokaryotes, a GTP molecule is needed in

• A. Formation of formyl-met-tRNA
• B. Binding of 30 S subunit of ribosome with mRNA
• C. Association of 30 S mRNA with formyl-met-tRNA
• D. Association of 50 S subunit of ribosome with initiation complex

Answer 1

Answer: (C)

Association of 30 S mRNA with formyl-met-tRNA

Translation is the process by which the m-RNA is translated into protein. it takes place in three major steps known as initiation, elongation, and termination. GTP act as an immediate source of energy which is hydrolyzed by GTPase.

The initiation of the polypeptide chain in prokaryotes is always brought about by the amino acid methionine but it has to be formulated to form formylmethionine (fmet). This methionine binds with tRNA-${}^{fmet}$ to form fmet-tRNA${}^{fmet}$

This fmet−tRNA${}^{fmet}$complex binds with the mRNA-30 S subunit complex using initiation factors IF-2 and IF-1 and GTP.