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B
We can break DNA at specific points by DNA-ases
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C
Availability of restriction endonucleases in purified form
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D
Knowledge of transduction
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Solution
The correct option is C Availability of restriction endonucleases in purified form Genetic engineering has been made possible due to availability of restriction endonucleases in purified form. Restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. In bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.