Question

How did the American Company, Eli Lily use the knowledge of r-DNA technology to produce human insulin? [3]

Answer 1

The American company, Eli Lily used the following technique to produce synthetic human insulin or humulin. The steps followed were :
Two DNA sequences corresponding to A and B chains of human insulin were prepared [1] and introduced into plasmids of E. coli. [1] Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin. [1]