CameraIcon

SearchIcon

MyQuestionIcon

3

You visited us 3 times! Enjoying our articles? Unlock Full Access!

Ligase

If a eukaryot...

Question

If a eukaryotic gene is incorrectly inserted into a plasmid vector, the recipient bacterium may fall to express the gene, because it cannot recognize the eukaryotic

A

DNA dependent RNA polymerase

No worries! We‘ve got your back. Try BYJU‘S free classes today!

B

Introns

No worries! We‘ve got your back. Try BYJU‘S free classes today!

C

mRNA codons

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

D

Start and stop punctuation signals

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is C mRNA codons
One way of detecting a specific cloned gene is by detecting its protein product expressed in the bacterial cell. In these cases, it is necessary to be able to express the gene in bacteria, that is, to transcribe it and translate the mRNA into protein. If a eukaryotic gene is incorrectly inserted into a plasmid vector the recipient bacterium may fail to express the gene because it cannot recognize the eukaryotic mRNA codons.
Thus, the correct answer is option C.

Suggest Corrections

thumbs-up

0

Similar questions

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Select the correct sequence of cloning method.

Q. The figure above illustrates gene expression in prokaryotic and eukaryotic cells:
Human insulin, a protein hormone that controls blood sugar levels, and many other eukaryotic proteins can be mass-produced by inserting eukaryotic genes into bacteria.
If an unaltered gene for human insulin was inserted into the DNA of a bacteria cell, how would the expression of the insulin gene be affected?

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except

Q. A biologist isolated a gene from a human cell, attached it to a plasmid and inserted the plasmid into a bacterium. The bacterium made a new protein, but it was nothing like the protein normally produced in a human cell. Why?

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

Tools of Recombinant DNA Technology

BIOLOGY

Watch in App

explore_more_icon

Explore more

Standard XII Biology

Join BYJU'S Learning Program

CrossIcon