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Question

If Meselson and Stahl's experiment is continued for three generations in bacteria. The ratio of 15N/15N:15N/14N:14N/14N containing DNA in the third generation would be

A
0:1:7
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B
1:4:0
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C
0:1:3
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D
0:1:4
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Solution

The correct option is C 0:1:3
Meselson and Stahl's experiment is based on the semi-conservative replication of the DNA. It means that when a parental DNA undergoes replication, each strand of the parental DNA serves as a template and the new daughter strand is synthesized on the template strand. Keeping the semi-conservative replication in view, and starting with DNA strands having 15N and thereafter 14N, the ratio comes out to be 0:1:3 as shown below. Hence, the correct answer is 0:1:3.


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