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Question

If Meselson and Stahl’s experiment is continued for 5 generations in bacteria, the ratio of 15N15N:15N14N:14N14N in 5th generation would be

A
0 : 1 : 3
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B
0 : 1 : 7
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C
0 : 1 : 15
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D
0 : 1 : 31
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Solution

The correct option is C 0 : 1 : 15
In 1st generation,
All DNA will be hybrid
Hence the ratio will be
0 Heavy : 1 Hybrid : 0 Light

In 2nd generation,
50 % would be Hybrid(15N14N) and 50 % would be Light (14N14N)
That is 0 Heavy : 1 Hybrid : 1 Light

In 3rd generation,
25 % would be Hybrid and 75 % would be Light DNA
0 Heavy : 1 Hybrid : 3 Light)

In 4th generation,
12.5 % would be Hybrid and 87.5 % would be Light DNA
(0 : 1 Hybrid : 7 Light)

In 5th generation,
6.25 % would be Hybrid and 93.75 % would be Light DNA
0 Heavy : 1 Hybrid : 15 Light).

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