Question

In DNA fingerprinting, the process of DNA hybridization with the help of a specific DNA probe is called ________.

• A. Western blotting
• B. Northern blotting
• C. Southern blotting
• D. All the above

Answer 1

Answer: (C)

Southern blotting

• Edward Southern in 1975 first developed the technique of southern blotting. It is used in DNA fingerprinting. The probes or markers that are radioactive synthetic DNA segments target a specific nucleotide sequence that is complementary to VNTRs sequences and hybridizes them.
• Western blotting is based on immunochromatography where proteins are separated into polyacrylamide gel based on their molecular weight.
  
• Northern blotting is conducted to detect RNA sequences,
  

Hence, the correct option is C.