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Question
In gel electrophoresis, the speed of migration of molecules (in their ionic form) in an electric field depends upon which of the following factor(s)?
In gel electrophoresis, the speed of migration of molecules (in their ionic form) in an electric field depends upon which of the following factor(s)?
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Solution
The correct option is D All the above
Voltage difference between two points creates an electric field between them. The electric field exerts a force on a charged particle present in that area. This creates the driving force of the charged particle to move. This is the principle on which the technique of gel electrophoresis is based upon.
The speed of migration of ions or charged species in an electric field is influenced by:
- Size of the molecule: Size is inversely proportional to the speed with which the molecule moves. A linear DNA molecule with lesser number of base pairs will encounter lesser frictional resistance in the agarose matrix and percolate swiftly through the pores than that with greater number of base pairs.
- Shape of the molecule: A more compact molecule will travel faster across the pores of a gel than a less compact molecule.
- Magnitude of charge: The rate of migration is directly proportional to the magnitude of charge. The greater the net charge, the faster the movement of the molecule.
Voltage difference between two points creates an electric field between them. The electric field exerts a force on a charged particle present in that area. This creates the driving force of the charged particle to move. This is the principle on which the technique of gel electrophoresis is based upon.
The speed of migration of ions or charged species in an electric field is influenced by:
- Size of the molecule: Size is inversely proportional to the speed with which the molecule moves. A linear DNA molecule with lesser number of base pairs will encounter lesser frictional resistance in the agarose matrix and percolate swiftly through the pores than that with greater number of base pairs.
- Shape of the molecule: A more compact molecule will travel faster across the pores of a gel than a less compact molecule.
- Magnitude of charge: The rate of migration is directly proportional to the magnitude of charge. The greater the net charge, the faster the movement of the molecule.
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