Inhibitory antigen is present in which of the following technique?
A
Direct ELISA
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B
Indirect ELISA
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C
Competitive ELISA
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D
All of the above
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Solution
The correct option is D Competitive ELISA The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. In competitive ELISA, the antigen in the sample and the antibody are incubated. The antigen-antibody mixture is added to the well in which there are other antigens. The previously bound antibodies will be washed off. The secondary antibody conjugated with the enzyme is used for the detection of the colour. The intensity of the colour is inversely proportional to the antigen which needs to be measured.