Question

Is EcoRI a restriction enzyme?

Answer 1

Restriction Enzyme:

1. The restriction enzyme is a protein produced by the bacteria that cleave DNA at specific sites.
2. The restriction enzymes recognize short and specific nucleotide sequences in DNA known as the recognition sequences.
3. Yes, EcoRI is known as a restriction enzyme or restriction endonuclease.
4. It is capable of cutting the DNA double helix at a specific site.
5. This restriction enzyme has been isolated from E.coli.
6. The restriction site of EcoRI is a palindromic sequence and it cuts DNA after G(Guanine) forming sticky at a sticky end with AATT(Adenine, adenine, thymine, thymine).